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Ideal Imaging for LED Light Engines with Fluorescence Microscopy

Posted by admin on August 5th, 2016

While LED light engines are becoming more and more popular for those using fluorescence microscopy, the performance of these engines are typically limited by standard filter sets. This even applies to those that are included with the sources. Fortunately, it is possible to achieve clean, bright images and it is not that hard to do. All you have to do is follow a few simple filter selection guidelines.

Improving the Industry

With commercial availability and performance improvements, LED based sources are replacing conventional gas-discharge lamps when it comes to fluorescence microscopy applications. The benefits of LED light engines of today include stable output, rapid wavelength switching, finely tuned output intensity and long life. The experts here at Data Display Products stress the importance of using the correct filters to produce high quality signal to noise ratio in single-molecule imaging applications.

Despite the light source type being used for experimental setups, the goal is to create images with the best quality. This means capturing images with minimal noise and bright signals. Thin-film optical filters are critical to optimal SNR. Integrated excitation filters are now available with LED light engines, which will narrow the output to include only wavelengths of the greatest intensity. However, additional optical filters should also be considered in the system.

Filters for Microscopy

In fluorescence microscopy, there are three primary filter types. These include a dichroic beamsplitter, an excitation filter and an emission filter. The light illuminating the sample is controlled by the excitation filter. Light at the desired wavelength is passed through to the sample, blocking light from any undesired wavelengths.

Similarly, an emission filter works to control the light that the detector records. This filter makes it possible for the right fluorescence signal from the sample to reach the detector, while blocking the light from any other wavelengths.

Finally, the dichroic beamsplitter is placed between the emission filter and the excitation filter. This will reflect the illumination that is emitted from the excitation filter. This light is directed to the sample and the light from the sample is also transmitted. It is then directed to the emission filter where it can be detected.

DDP Engineered LED Solutions is a leader in LED light engines manufacturing. We work hard to not only meet, but exceed the expectations of every customer. Give us a call today to find out how we can provide you with the best lighting solutions for your application.

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